Tifs in endocytosis. Either or both NPF motifs were mutated to NAA to reduce the interaction with the EH domain of the scaffold proteins Pan1 and Ede1 (de Beer et al., 1998; Aguilar et al., 2003). ENTH1 cells were transformed with overexpression plasmids encoding the single Ent1C-termNPF1- or Ent1C-termNPF2- mutants, or the Ent1C-termNPF- double mutant, and growth and endocytosis phenotypes tested