Wing day using a standard calcium phosphate precipitation method or via FuGENE (Roche). Empty control plasmids were added to ensure that each transfection received the same amount of total DNA. To normalize the transfection efficiency, pRL-TK Renilla luciferase reporter plasmids were added to each transfection. The luciferase assays were performed using a Dual-Specific Luciferase Assay kit (Promeg