Ompare P2-silenced cells transfected with mock, 16.1 1.0 vs. P2-ECFP, 22.2 1.6 mol O2/min/106 cell, p 0.001) (Figure 8A). BN-PAGE demonstrated that complex IV assembly was normalized in P2silenced cells stably expressing P2-ECFP (Figure 8B). The second dimension of the BN-PAGE showed that P2-ECFP expression prevented the accumulation of complex IV assembly intermediates, upon P2 silencing (compare