Diacylglycerol-type to ceramide-type in the ER (IPC/B) and Golgi (IPC/C; Reggiori et al., 1997; Sipos et al., 1997). In the gup1 per1 double-mutant cells, we detected significant amounts of IPC/C in addition to PI, indicating that a small number of conventional diacylglyceroltype of GPI-anchored proteins could be replaced by ceramide-type GPI-anchored proteins in the Golgi of these cells. Gas1p co