Anada).Native ImmunoprecipitationHA-fusion proteins and FLAG-fusion proteins were immunoprecipitated from the extracts prepared as above by incubating with anti-HA-agarose and with anti-FLAG M2-agarose (Sigma-Aldrich, St. Louis, MO), respectively, for 2 h at 4 . After five-times wash with lysis buffer, protein-RNA complexes were eluted by boiling in 25 mM Tris-HCl, pH 7.5, 10 mM EDTA, and 1.0 SDS