Late trophozoite stage (40 hpi) when the parasite is metabolically most active. However, PfEK and PfEP showed almost similar levels in all the stages (with a slight increase in the trophozoite/schizont stages) suggesting their near constitutive expression (Fig. 2a). Detection of Selected ES Antigens in Parasite Culture Supernatant by Western Blotting--To confirm that the selected proteins are expo