Ying SNARE complex assembly and disassembly and their control by chaperones including membrane tethering factors, Sec1-Munc18 proteins (SM proteins) [G], and Nethylmaleimide-sensitive fusion factor (NSF) [G]. Membrane tethering factors8-10 not only mediate the initial contact between a vesicle and its target membrane but also bind SNAREs to regulate their assembly. Tethering factors work together