S three.0 and Biotools 3.1 sw have been applied to generate the fragments mass list and also the sequence tag of peptide. The mass list was sent to database in similar way as was performed in case of above PMF method. The mass tolerance error of 0.5 Da to 1.0 Da was made use of for MSMS ion search. The MSMS ion search confirmed the protein identity and provided the amino acid sequence of certain p