Nds on agarose gel was quantified working with Quantity One Application BioRad, Gel Doc Technique. We further selected two proteins 3hydroxyanthranilate three, 4dioxygenase, Haao and Cytochrome b5a, Cyb5a around the basis of their expression level and physiological roles in hypoxia for validation. Lungs type all of the experimental groups have been perfused, fixed, removed and further fixed in ten