Easuring absorbance at 260 nm along with the purity was assessed by the A260280 ratio. Samples with A260280 ratio between 1.eight and two.0 were taken for further proceedings. Total isolated RNA was reverse transcribed applying Enhanced AMV Reverse Transcriptase Enzyme technique Sigma, St Louis, MO in line with the producers protocol. Briefly, total RNA 1 g was mixed with 500 M dNTPs, two.5 M r