NM Okadaic Acid, and 1 HALT phosphatase inhibitor mixture (Pierce, Rockford, IL)). Thirty g of cleared whole cell lysates (quantified using the Bradford assay) was resolved on 10 Tris-Glycine SDS-PAGE. Manganese and 5 U of lambda phosphatase (New England Biolabs, Ipswich, MA) were added to whole cell lysates as indicated and the reaction was incubated at 30 for 30 min; stopped with Laemmli prote