Cript RT-PCR system (Invitrogen). Template cDNA was added to Taqman Universal Master Mix (Applied Biosystems, Foster City, CA) in a 15- l reaction with specific primers and probes acquired from Applied Biosystems (Foster City, CA) for KiSS-1 (Hs_00158486_m1) and the chaperonin containing T-complex protein 1 subunit 6A ( 1) (CCT6A) (Hs_00798979_s1). Quantification of gene expression was carried out