Beled hemocyte protein samples were acidified with 1 M phosphoric acid to a pH less than 3.0 and then separated on a Hewlett Packard 1090 HPLC system fitted with a polySulfoethylA column (The Nest Group, Southborough, MA). At 0.5 ml/min of buffer A (10 mM KH2PO4, pH 3.0, 25 acetonitrile), a gradient of 0 to 100 buffer B (10 mM KH2PO4, 1 M NaCl, pH 3.0, 25 acetonitrile) was established over 120