Tained after transient transfection of ApoE isoforms provided evidence of association between ApoE isoforms and RelA (Fig. 7B). Thus, although RelA coimmunoprecipitated with both ApoE3 and ApoE4, only ApoE4 led to the translocation of RelA into the nucleus. We also evaluated the interaction of ApoE and the putative RelA-binding region, acgtggaaattccca, on the SirT1 promoter by SPR. Neither ApoE is
Of neurite growth (Table 1). (A) O14782 (Kif3C) is an example of very early protein association with the sites of neurite outgrowth. (B) Q8TBG9 (synaptoporin) is an example of later association with the neurite tip. Proteins that blocked neurite outgrowth were classified regarding the dynamics of neurite growth (Table 3) (C) Q9UKI2 (Borg2) is an example where neurites never develop. (D) Q9BQI0 (hy
Of neurite growth (Table 1). (A) O14782 (Kif3C) is an example of very early protein association with the sites of neurite outgrowth. (B) Q8TBG9 (synaptoporin) is an example of later association with the neurite tip. Proteins that blocked neurite outgrowth were classified regarding the dynamics of neurite growth (Table 3) (C) Q9UKI2 (Borg2) is an example where neurites never develop. (D) Q9BQI0 (hy
Tifs in endocytosis. Either or both NPF motifs were mutated to NAA to reduce the interaction with the EH domain of the scaffold proteins Pan1 and Ede1 (de Beer et al., 1998; Aguilar et al., 2003). ENTH1 cells were transformed with overexpression plasmids encoding the single Ent1C-termNPF1- or Ent1C-termNPF2- mutants, or the Ent1C-termNPF- double mutant, and growth and endocytosis phenotypes tested