NM Okadaic Acid, and 1 HALT phosphatase inhibitor mixture (Pierce, Rockford, IL)). Thirty g of cleared whole cell lysates (quantified using the Bradford assay) was resolved on 10 Tris-Glycine SDS-PAGE. Manganese and 5 U of lambda phosphatase (New England Biolabs, Ipswich, MA) were added to whole cell lysates as indicated and the reaction was incubated at 30 for 30 min; stopped with Laemmli prote
Nce for the novel object ( p 0.3). A significant drug effect was observed at this dose ( p 0.02 compared with vehicle). At lower doses (5?0 g/kg), no drug effect was observed (n 6 ?). We then assessed the effect of the SST3 antagonist when injected after the sample phase, 30 min before the test phase, again using a 1 h retention interval (Fig. 6 B). Performance of mice in discriminating between th
E used as controls. Oligonucleotides were delivered by standard lipofection and 48 h after transfection, subunit c isoforms silencing efficiency was estimated by qRT-PCR. The silencing of individual subunit c isoforms resulted in a variable reduction of the corresponding mRNA levels ranging from 80 to 95 of scrambled controls (Figure 1B). The silencing of P1 and P2 was also associated with a mode
E used as controls. Oligonucleotides were delivered by standard lipofection and 48 h after transfection, subunit c isoforms silencing efficiency was estimated by qRT-PCR. The silencing of individual subunit c isoforms resulted in a variable reduction of the corresponding mRNA levels ranging from 80 to 95 of scrambled controls (Figure 1B). The silencing of P1 and P2 was also associated with a mode