Of neurite growth (Table 1). (A) O14782 (Kif3C) is an example of very early protein association with the sites of neurite outgrowth. (B) Q8TBG9 (synaptoporin) is an example of later association with the neurite tip. Proteins that blocked neurite outgrowth were classified regarding the dynamics of neurite growth (Table 3) (C) Q9UKI2 (Borg2) is an example where neurites never develop. (D) Q9BQI0 (hy
Of neurite growth (Table 1). (A) O14782 (Kif3C) is an example of very early protein association with the sites of neurite outgrowth. (B) Q8TBG9 (synaptoporin) is an example of later association with the neurite tip. Proteins that blocked neurite outgrowth were classified regarding the dynamics of neurite growth (Table 3) (C) Q9UKI2 (Borg2) is an example where neurites never develop. (D) Q9BQI0 (hy
Tifs in endocytosis. Either or both NPF motifs were mutated to NAA to reduce the interaction with the EH domain of the scaffold proteins Pan1 and Ede1 (de Beer et al., 1998; Aguilar et al., 2003). ENTH1 cells were transformed with overexpression plasmids encoding the single Ent1C-termNPF1- or Ent1C-termNPF2- mutants, or the Ent1C-termNPF- double mutant, and growth and endocytosis phenotypes tested
Tained after transient transfection of ApoE isoforms provided evidence of association between ApoE isoforms and RelA (Fig. 7B). Thus, although RelA coimmunoprecipitated with both ApoE3 and ApoE4, only ApoE4 led to the translocation of RelA into the nucleus. We also evaluated the interaction of ApoE and the putative RelA-binding region, acgtggaaattccca, on the SirT1 promoter by SPR. Neither ApoE is
G Technology) at 1:1,000) in 2.5 milk. The next day, the membrane was rinsed with Tris-buffered saline (137 mM NaCl, 2.7 mM KCl, 19 mM Tric Base, pH 7.4) containing 0.1 Tween-20 and incubated with secondary anti-rabbit and anti-mouse antibodies (1:20,000 for both) conjugated with horseradish peroxidase. Immunoblots were visualized by chemiluminescence (GE Healthcare) and recorded on Fujifilm LAS
G Technology) at 1:1,000) in 2.5 milk. The next day, the membrane was rinsed with Tris-buffered saline (137 mM NaCl, 2.7 mM KCl, 19 mM Tric Base, pH 7.4) containing 0.1 Tween-20 and incubated with secondary anti-rabbit and anti-mouse antibodies (1:20,000 for both) conjugated with horseradish peroxidase. Immunoblots were visualized by chemiluminescence (GE Healthcare) and recorded on Fujifilm LAS
G Technology) at 1:1,000) in 2.5 milk. The next day, the membrane was rinsed with Tris-buffered saline (137 mM NaCl, 2.7 mM KCl, 19 mM Tric Base, pH 7.4) containing 0.1 Tween-20 and incubated with secondary anti-rabbit and anti-mouse antibodies (1:20,000 for both) conjugated with horseradish peroxidase. Immunoblots were visualized by chemiluminescence (GE Healthcare) and recorded on Fujifilm LAS
Including AACT, appear to promote tumor progression15. It was shown that tumors from a metastatic breastCancer. Author manuscript; available in PMC 2013 January 15.Roberts et al.Pagecancer cell line had higher levels of expression of AACT than those produced from a nonmetastatic cell line16. Additionally, malignant breast tumor cells induced local AACT expression by host cells at primary and secon