Y 1.Raedler et al.Pagerat anti-mouse IL-17A (clone TC11-8H4). After washing, an alkaline phosphataseconjugated anti-biotin antibody (Vector Laboratories, Burlingame, CA) diluted 1:2000 in PBS supplemented with 0.1 Tween and 1 bovine serum albumin (BSA) was added for 90 min, the plates were developed by addition of 1-Step NBT/BCIP substrate (Thermo Scientific, Rockland, IL), and the resulting spot