Transferase 1A1. one hundred L of the diluted plasma samples have been added onto 96 properly plate and incubated at 37 for 2 h. A 100 L of biotinylated antibody was added, mixed and incubated for 1 h at 37 . Just after 3 washes with wash buffer, one hundred L of HRP vidin 1x was added and incubated for 1 h at 37 . Right after 5 washes, 90 L of TMB substrate was added and incubated for 150