http://64188.org/story.php?title=transferase-1a1-one-hundred-l-from-the-diluted-plasma-samples-were-added-onto Transferase 1A1. one hundred L of the diluted plasma samples have been added onto 96 properly plate and incubated at 37 for 2 h. A 100 L of biotinylated antibody was added, mixed and incubated for 1 h at 37 . Just after 3 washes with wash buffer, one hundred L of HRP vidin 1x was added and incubated for 1 h at 37 . Right after 5 washes, 90 L of TMB substrate was added and incubated for 150 Fri, 07 Jul 2023 04:03:21 UTC en