S three.0 and Biotools 3.1 sw were applied to generate the fragments mass list and also the sequence tag of peptide. The mass list was sent to database in very same way as was performed in case of above PMF approach. The mass tolerance error of 0.5 Da to 1.0 Da was utilized for MSMS ion search. The MSMS ion search confirmed the protein identity and offered the amino acid sequence of certain peptid