Gel tryptic peptides of each spot ofinterest had been mixed with an acidic solid matrix for example cyano4hydroxy cinnamic acid CHCA, Bruker Daltonics matrix 10 mgml, which delivers high sensitivity and negligible matrix adduction throughout the laser absorption and subjected to laser radiation. The matrix was made in 70 acetonitrile and 0.03 TFA. 0.5 l on the peptide extracts mixed together w