Ody production did not appear to participate in the induction of IgG since neither IL-4 nor IL-21 cytokines were detected in the supernatants of these co-cultures (data not shown). More importantly, IgG subclass profiling revealed that the main IgG subclasses produced by DNA-DC-activated B cells were the effector IgG1 and IgG3 isotypes, which are known potent activators of the complement system, c
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