Have been added to cover gel pieces for shrinking the gel pieces. The gel pieces were rehydrated in ten mM NH4HCO3 for five min, equal volume of acetonitrile were added and removed following 15 min of incubation. The gel pieces have been once again covered with acetonitrile and removed. The gel pieces had been dried inside a vacuum centrifuge. The dried gel pieces have been digested with 20 ul of