E location of the hybridized segments on the DNA [29 . Despite these significant challenges, nanopore-based sequencing remains compelling owing to the prospects of: (i) inexpensive sample preparation [29?282], (ii) small sample requirement of only 108 copies of DNA (a number that can be obtained without amplification) [29?283], (iii) potentially long-read lengths of several kilobases [29? 222,282
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