Cytoplasm in HEK293T cells (Fig. 4C). Second, we expressed NanoLuc?tagged TNIP2 (or TNIP2 Tyr230Ala as a control), together with fluorescently labeled TSG101-Halo (or unlabeled TSG101-Halo as a control), and tested for bioluminescence resonance energy transfer (NanoBRET) in HEK293T cells (42). We detected similar levels of donor luminescence for NanoLuc?TNIP2 and NanoLuc?TNIP2 Tyr230Ala samples (F