Here to glass coverslips for 30 min (all steps were carried out at room temperature). After attachment, the hemocytes were rinsed two times in Squid Ringer's to remove excess tissue or cellular debris and then fixed with 4 paraformaldehyde in Squid Ringer's for 30 min. After fixation, the hemocytes were washed four times with marine phosphate-buffered saline (mPBS), pH 7.4 (50 mM sodium phosphate
Ed our knowledge about organogenesis and organ cellular composition. Here, we present an atlas of the cell-type-resolved liver proteome. In-depth proteomics identified 6000 to 8000 gene products (GPs) for each cell type and a total of 10,075 GPs for four cell types. This data set revealed features of the cellular composition of the liver: (1) hepatocytes (PCs) express the least GPs, have a unique