Here to glass coverslips for 30 min (all steps were carried out at room temperature). After attachment, the hemocytes were rinsed two times in Squid Ringer's to remove excess tissue or cellular debris and then fixed with 4 paraformaldehyde in Squid Ringer's for 30 min. After fixation, the hemocytes were washed four times with marine phosphate-buffered saline (mPBS), pH 7.4 (50 mM sodium phosphate