As hepatosplenomegaly. The maximum diameter of the paraaortic lymph nodes was 8 cm (Fig. 2A). We diagnosed a relapse of CLL based on the increased lymphocyte count and sIL-2R level in peripheral blood. As the patient's disease was refractory to the previous chemotherapies, bendamustine was administered at a dose of 70 mg/m2/day for 2 days and this was repeated on days 42?3, 98?9, and 182?83 [19] w
Nan (100 /ml) for 3 h at 37 in a 5 CO2 atmosphere. Cells were washed four times and autologous stimulated T cells were added onto HIV-exposed MDDC at a DC:T-cell ratio of 1:5. Each sample was performed in triplicate. Culture supernatants were harvested every 3 days and fresh medium was added. Supernatants were inactivated with 1 Triton X-100 and frozen at -20 . The viral production by T lympho
Ch lectin to interact with epithelial cell's receptors, labeled HHA and GNA were incubated with epithelial cells. As depicted in Figure 2C, only HHA but not GNA interacted with receptors expressed at the surface of HEC-1. Moreover, the preincubation of GNA with epithelial cells followed with several washes did not inhibit viral attachment whereas the preincubation of GNA with R5 viral particles in
As hepatosplenomegaly. The maximum diameter of the paraaortic lymph nodes was 8 cm (Fig. 2A). We diagnosed a relapse of CLL based on the increased lymphocyte count and sIL-2R level in peripheral blood. As the patient's disease was refractory to the previous chemotherapies, bendamustine was administered at a dose of 70 mg/m2/day for 2 days and this was repeated on days 42?3, 98?9, and 182?83 [19] w
Plant lectins. The HIV-1J-RCSF strain was used in this experiment due to the low transcytosis ability of HIV-1Ba-L strain [8]. As shown in Figure 3A, HHA inhibited transcytosis of cell-free HIV-1 in a dose-dependent manner. Similarly to mannan (100 /ml) that inhibited HIV transcytosis up to 41 , increasing concentrations of HHA (range 1-10-100 /ml) afforded a 17 , 42 and 54 decrease of HIV-1JR
Achment on HEC-1A Considering that both HHA and GNA were non-toxic in the epithelial cell system, we further investigated the inhibition of HIV adsorption on the apical side of HEC-1A by the compounds. Cells were thus incubated with HIV-1Ba-L in the presence or absence of different concentrations of HHA or GNA. As depicted in Figure 2A, HHA faintly decreased the attachment of HIV-1Ba-L on epitheli
Nan (100 /ml) for 3 h at 37 in a 5 CO2 atmosphere. Cells were washed four times and autologous stimulated T cells were added onto HIV-exposed MDDC at a DC:T-cell ratio of 1:5. Each sample was performed in triplicate. Culture supernatants were harvested every 3 days and fresh medium was added. Supernatants were inactivated with 1 Triton X-100 and frozen at -20 . The viral production by T lympho
Tration of the study plant lectins in epithelial cell (A). MDM)line (HEC-1A) and primary immune cells (MDDC (A). Non-toxic concentration of the study plant lectins in epithelial cell line (HEC-1A) and primary immune cells (MDDC and MDM). HEC-1A and primary immune cells were cultured with concentrations of products for 24 h. After washing, culture viability was determined by using the MTT-cytotoxic