With YFP-G i2 and Ric-8A Cherry, thatmcb.asm.orgMolecular and Cellular BiologyG-Protein Signaling and PhagocytosisFIG 6 Altered phagocytosis in BMDM prepared from Gnai3 / , Gnai2 / , and Gnai2G184S/G18S mice. (A) Confocal microscopy of WT and Gnai3 / BMDM exposed to fluorescent zymosan (green) for 15 min prior to fixation and staining with phalloidin (red). Bar, 20 m. (B) ImageStream analysis of W
Nductance of these pores. [PubMed: 87221] 192. Macrae, MX.; Blake, S.; Mayer, T.; Mayer, M.; Yang, J. Reactive derivatives of gramicidin enable light-and ion-modulated ion channels. Proceedings of the Society of Photo-Optical Instrumentation Engineers; 2009. p. 739709 193? Futaki S, Zhang YJ, Sugiura Y. Detecting a tag on a channel opening: blockage of the biotinylated channels by streptavidin. Te
Nductance of these pores. [PubMed: 87221] 192. Macrae, MX.; Blake, S.; Mayer, T.; Mayer, M.; Yang, J. Reactive derivatives of gramicidin enable light-and ion-modulated ion channels. Proceedings of the Society of Photo-Optical Instrumentation Engineers; 2009. p. 739709 193? Futaki S, Zhang YJ, Sugiura Y. Detecting a tag on a channel opening: blockage of the biotinylated channels by streptavidin. Te
Xpressed proteins. Functional Validation of Interactions by shRNA-Mediated Knockdown--To determine the extent to which the high confidence interactors identified by I-DIRT play a role in DENV infection, we used shRNA to silence a panel of host genes and then determined the effect on DENV infection. The genes in our panel included interactors of both NS5-GFP and NS3GFP (CAD, DHCR7), only NS5-GFP (M
Xpressed proteins. Functional Validation of Interactions by shRNA-Mediated Knockdown--To determine the extent to which the high confidence interactors identified by I-DIRT play a role in DENV infection, we used shRNA to silence a panel of host genes and then determined the effect on DENV infection. The genes in our panel included interactors of both NS5-GFP and NS3GFP (CAD, DHCR7), only NS5-GFP (M
Xpressed proteins. Functional Validation of Interactions by shRNA-Mediated Knockdown--To determine the extent to which the high confidence interactors identified by I-DIRT play a role in DENV infection, we used shRNA to silence a panel of host genes and then determined the effect on DENV infection. The genes in our panel included interactors of both NS5-GFP and NS3GFP (CAD, DHCR7), only NS5-GFP (M
Dissolved in 1 acetic acid for LC-MS analysis. The resulting tryptic peptide mixtures were subjected to nano-liquid chromatography coupled to mass spectrometry for protein identification. Peptides were injected onto a C-18 reversed phase (RP) nano-column (100 m I.D. and 12 cm, Mediterranea Sea, Teknokroma) and analyzed in a continuous acetonitrile gradient consisting of 0 ?0 B in 140 min, 50 ?0