Dissolved in 1 acetic acid for LC-MS analysis. The resulting tryptic peptide mixtures were subjected to nano-liquid chromatography coupled to mass spectrometry for protein identification. Peptides were injected onto a C-18 reversed phase (RP) nano-column (100 m I.D. and 12 cm, Mediterranea Sea, Teknokroma) and analyzed in a continuous acetonitrile gradient consisting of 0 ?0 B in 140 min, 50 ?0