Ndividually in "live-gate" mode. The results were expressed as relative fluorescence intensity (RFI) calculated as geometric mean fluorescence intensity (GFI) of stimulated cells/GFI of control (unstimulated) cells. The experiment was repeated three times and the expression levels were expressed as the mean SEM. Statistical significance was determined using the Student's t test.RESULTS Membrane Ru
Ndividually in "live-gate" mode. The results were expressed as relative fluorescence intensity (RFI) calculated as geometric mean fluorescence intensity (GFI) of stimulated cells/GFI of control (unstimulated) cells. The experiment was repeated three times and the expression levels were expressed as the mean SEM. Statistical significance was determined using the Student's t test.RESULTS Membrane Ru
Tudied member of the Lrp family (241) or the FFRP (feast/famine regulatory protein) family (242). Because many reviews (6, 241?244) dealing with Lrp can be found in the literature, we only summarize here. Lrp is a small protein ( 19 kDa) (244). It consists of an N-terminal DNA binding domain, harboring the canonical helix-turnhelix motif, connected with a flexible hinge to a C-terminal ligand bind
Ase their cargo (53). Secretionof exosomes has been associated to situations requiring communication between cells like antigen presentation, or to pathological conditions like cancer cell signaling (41, 53). According to ExoCarta analysis of our data, exosomal protein secretion was activated in MSU-stimulated human macrophages and is the major secretory pathway activated by MSUMolecular Cellula
Um (109). Several determinants were identified which either localize to the spore (based on gene fusion studies) or are required for full BclA assembly. These may represent either exosporium proteins or possibly nonspore proteins that participate in exosporium assembly.FUNCTIONS OF THE EXOSPORIUM IN THE ENVIRONMENTGerQ is an exosporium protein first characterized in B. cereus and B. thuringiensis
Ple Preparation. Intra-assay precision was defined as the peptide concentration CV for the three runs on the same day and then averaged across the 3 days. The inter-assay precision was defined as the peptide concentration CV for the same run on 3 days (e.g. Day 1, Run 1; Day 2, Run 1; and Day 3, Run 1) and then averaged across the 3 runs. This experiment was performed for 6 individual fresh whole-
Ple Preparation. Intra-assay precision was defined as the peptide concentration CV for the three runs on the same day and then averaged across the 3 days. The inter-assay precision was defined as the peptide concentration CV for the same run on 3 days (e.g. Day 1, Run 1; Day 2, Run 1; and Day 3, Run 1) and then averaged across the 3 runs. This experiment was performed for 6 individual fresh whole-
Ple Preparation. Intra-assay precision was defined as the peptide concentration CV for the three runs on the same day and then averaged across the 3 days. The inter-assay precision was defined as the peptide concentration CV for the same run on 3 days (e.g. Day 1, Run 1; Day 2, Run 1; and Day 3, Run 1) and then averaged across the 3 runs. This experiment was performed for 6 individual fresh whole-