Hat both the mutant and wild-type Pol bound to monoubiquitinated PCNA in BPDE-treated cells, indicating that at least one of the two ubiquitin-binding domains (designated UBZs) was involved in the binding. This result eliminates a trivial explanation that the reduced REV1-binding activity of the Pol mutant protein is due to failure in protein folding. Thus, we conclude that the interaction with RE
Ene contexts. For Type II molecules, variability of interactants found in individual experiments for such proteins is overcome by analyzing large single IP/MS studies like the one presented here. ItCell. Author manuscript; available in PMC 2012 May 27.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptMalovannaya et al.Pagethen becomes clear that a protein that biochemically
Ene contexts. For Type II molecules, variability of interactants found in individual experiments for such proteins is overcome by analyzing large single IP/MS studies like the one presented here. ItCell. Author manuscript; available in PMC 2012 May 27.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptMalovannaya et al.Pagethen becomes clear that a protein that biochemically
The protein among evolutionarily distant species and highlights Dictyostelium as a valid experimental system to address the function of this gene. Dictyostelium Vmp1 is an endoplasmic reticulum protein necessary for the integrity of this organelle. Cells deficient in Vmp1 display pleiotropic defects in the secretory pathway and organelle biogenesis. The contractile vacuole, which is necessary to s
The protein among evolutionarily distant species and highlights Dictyostelium as a valid experimental system to address the function of this gene. Dictyostelium Vmp1 is an endoplasmic reticulum protein necessary for the integrity of this organelle. Cells deficient in Vmp1 display pleiotropic defects in the secretory pathway and organelle biogenesis. The contractile vacuole, which is necessary to s
Ing inhibitor yd47 prevents biofilm formation in vitro and OM in a guinea pig model.87 Macrolides and fluoroquinolones are the most effective antibiotics for disrupting biofilms in vitro.88 The sensitizing milk protein HAMLET increases the potency of antibiotics to both sensitive and resistant pneumococcal strains.89 Complement: Phosphoglycerate kinase and elongation factor Tu were shown to be pne
D to zymosan focusing on a phagocytic cup. Numbers represent elapsed time (in minutes). For each time point, a bright-field overlay image and two fluorescent images electronically zoomed 2 are shown. Bar, 5 m. (B) Time-lapse confocal microscopy images of RAW 264.7 cells expressing YFP-G i2 (green) and RFP-Lifeact (red) and exposed to zymosan. Arrowheads indicate G i together with actin condensed a
D to zymosan focusing on a phagocytic cup. Numbers represent elapsed time (in minutes). For each time point, a bright-field overlay image and two fluorescent images electronically zoomed 2 are shown. Bar, 5 m. (B) Time-lapse confocal microscopy images of RAW 264.7 cells expressing YFP-G i2 (green) and RFP-Lifeact (red) and exposed to zymosan. Arrowheads indicate G i together with actin condensed a