Each V3 position. The position-specific error rates were then defined as the upper limit of the 99 confidence interval of the mean frequency of artifactual codons among the 20 clones at each position of V3. We then determined weighted error rates to construct a sensitivity threshold matrix at each position of V3 to identify authentic virus variants harboring a point mutation for a given number of
F (t)=E0 (0) ??For any single epitope, the probability of it fusing with the target cell it is bound to before it is disabled by a bound antibody is equal to f (?). The probability of this fusion being prevented by an antibody is pb 1{f (?) ??The probability pb of disabling a gp41 trimer in the pre-hairpin intermediate may depend on both A, the mAb concentration and on N, the number of gp41 trime
F (t)=E0 (0) ??For any single epitope, the probability of it fusing with the target cell it is bound to before it is disabled by a bound antibody is equal to f (?). The probability of this fusion being prevented by an antibody is pb 1{f (?) ??The probability pb of disabling a gp41 trimer in the pre-hairpin intermediate may depend on both A, the mAb concentration and on N, the number of gp41 trime
Becomes independent of N. We first calculate pb in this limit when there is a uniform distribution of gp41 trimers bridging the virus and target cell and pb is independent of N. When we analyze neutralization experiments we will consider how pb depends on N when N is small. To obtain an expression for pb , we assume the following: 1. In the absence of mAb or Fab bound to the MPER, fusion is irreve
B, and replotted the data as a function of A=IC50 , the data did lie on a universal curve (Figure 7A). From the analysis of these data we could not determine the number of gp41 bridging the virus and target cell at the start of the experiment, but the analysis indicated that these bonds are few in number and under considerably more strain then if there were a large number of attachments in the con
B, and replotted the data as a function of A=IC50 , the data did lie on a universal curve (Figure 7A). From the analysis of these data we could not determine the number of gp41 bridging the virus and target cell at the start of the experiment, but the analysis indicated that these bonds are few in number and under considerably more strain then if there were a large number of attachments in the con
L V3 variants of 20 virus clones. The position mean (red line) is the error rate estimated at each position of V3 by comparing the UDS reads to the Sanger sequences of 20 clones. The shaded regions represent the 99 confidence interval of global (blue) and position (red) mean error rates.compare the systems. The mean frequency of V3 variant artifacts found with the 454 GS-Junior was 0.018 [exact
Nding gendered influences on women's reproductive health in Pakistan: moving beyond the autonomy paradigm. Soc. Sci. Med., 68(7), 1349-56. http://dx.doi.org/10.1016/j.socscimed.2009.01.025 National Institute of Population Studies (NIPS) Islamabad Pakistan Macro International Inc. USA (June 2008). Pakistan Demographic and Health Survey 2006-07. Patton, M. Q. (1999). Enhancing the quality and credib