L V3 variants of 20 virus clones. The position mean (red line) is the error rate estimated at each position of V3 by comparing the UDS reads to the Sanger sequences of 20 clones. The shaded regions represent the 99 confidence interval of global (blue) and position (red) mean error rates.compare the systems. The mean frequency of V3 variant artifacts found with the 454 GS-Junior was 0.018 [exact