S. HPLC. When comparing the six NNK libraries, the ones primarily based on primers from supplier three exhibited the highest mean Qpool worth, when compared with suppliers 1 and two 0.738 vs. 0.603 and 0.507, respectively. For that reason, we utilized only primers from supplier 3 to evaluate the 3 other randomization schemes NNS, Tang and 22ctrick.sequencing results, a widespread finding observed