Patches was analyzed using Slide-Book 4.2 software (n 350 patches for each strain). Comparisons of the localization and patch intensity for Ede1-RFP versus Ede1EH--RFP can be found in Supplemental Figure 6, A and B. (C) Ede1 is required for viability at 26 in ENTH1 cells. Quadruple deletion cells ( ) (BWY2596 and BWY2597) and quintuple deletion cells (5 ) with an ENT1 TRP1 or an ENTH1 TRP1 plasmi