Onding glycoprobe-Sepharose microcolumn and incubated in 100 l HEPES running buffer (10 mM HEPES, 150 mM NaCl, 5 mM CaCl2 and 1 mM MnCl2, pH 7.4) for 24 h at 37 . Unbound material was removed by extensive washing with running buffer, then bound lectin was eluted with 0.1 TFA in 2:1 (v/v) ACN:H2O, except for Sia-containing columns (3 -SLN and SLex glycoprobes), for which a second competitive elut