Least three independent replicates of each experiment. All of the peptide and protein information acquired in this study is provided in supplemental Table S2. Construction of -lactamase TEM-1 Fusions--N-terminal translational fusions to TEM-1 -lactamase were generated in the vector pCX341 (provided by I. Rosenshine, Hebrew University, Jerusalem, Israel) (31, 32). The coding regions, without the st