Eomics 8.Cartilage Response to Mechanical Injury and Cytokinesregion ( 214 nm). Fractions were concentrated by SpeedVac and desalted by ZiptipTM (Millipore) to obtain 45 fractions for LC/ MS/MS analysis. LC/MS/MS--Fractions were injected manually (Rheodyne; manual injector with 0.5- l internal loop) onto a 75- m inner diameter 160 mm column with 10- m tip (New Objective) packed in-house with Vydac