Easuring absorbance at 260 nm plus the purity was assessed by the A260280 ratio. Samples with A260280 ratio involving 1.eight and two.0 had been taken for additional proceedings. Total isolated RNA was reverse transcribed applying Enhanced AMV Reverse Transcriptase Enzyme system Sigma, St Louis, MO in line with the makers protocol. Briefly, total RNA 1 g was mixed with 500 M dNTPs, 2.five M ran