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Condition. In total,54 sample injections (12 fetuin glycoprobe elutions, 24 standard glycoprobe elutions and 18 blank elutions) per each sperm condition (CM and NCM) were performed for MS/MS analysis of CREDEX experiments. In order to minimize instrumental variability, a defined batch file was programmed for sample injection. Specifically, samples were injected in groups of nine including the six
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