Any standard, those points were dropped. The curve fit parameter was altered if necessary depending on the number of standard points. 2.2. Flow cytometry Whole blood was collected into ethylenediaminetetraacetic acid (EDTA) anticoagulant and assayed within 24 h. Samples were processed using a simple lyse-wash-stain-wash technique. For a given sample, sixteen 5-mL polystyrene tubes were each filled