And from cells expressing Tat protein, 48 h after electroporation. qPCR was performed in the presence of SyBr Green (Sigma). Primer sequences and positions are in Table S1. Construction of stably transfected cell lines LMP vectors (Open Biosystems) expressing two microRNA-adapted shRNAs (shRNAmir) against the ER and a control shRNAmir (Table S1) were transfected into Phoenix cells with FuGENE6 rea