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Ent (Pharmacia). Isolation of PBLs, their activation with PHA/IL-2 and infection with HIV-1LAI followed by isolation of resting CD4+ T cells by negative selection using magnetic beads (Invitrogen) was performed as previously described (Contreras et al., 2007). Resting CD4+ T cells were treated with 1mM HMBA and 10 ng/ml TNF- for 24 h. Viral release in the supernatant was quantified by p24 ELISA (P
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Arriage, men's extramarital, sex, and HIV risk in South Eastern Nigeria. Am J Public Health 2007, 97:997-1005. Desilva MB, Merry SP, Fischer PR, Rohrer JE, Isichei CO, Cha SS: Youth, unemployment, and male gender predict mortality in AIDS patients started on HAART in Nigeria. AIDS Care 2009, 21:70-77.42. Oyediran K, Isiugo-Abanihe UC, Feyisetan BJ, Ishola GP: Prevalence of and factors associated w
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Explored, Cao et al. focused on perceived organizational help.They determinedExplored, Cao et al. focu
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Sted OR (AOR)=3.12, 95 CI (1.90 to 5.13), family physiciandominant co-management AOR=3.39, 95 CI (1.94 to 5.93), specialist-dominant co-management AOR=2.01, 95 CI (1.23 to 3.26)). The odds of having one emergency department visit did not differ among models, although the odds of hospitalisation and HIVspecific hospitalisation were lower among patients who saw exclusively family physicians (AOR=
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Ost Microbe. Author manuscript; available in PMC 2014 November 03.Lenasi et al.PageWestern blotting 107 J-Lat or J-Lat shER cells were lysed in 0.8 ml of lysis buffer A (10 mM Tris-HCl [pH 7.4], 150 mM NaCl, 2 mM EDTA, 1 NP-40, 0.1 protease inhibitor) for 40 min at 4 . Proteins in the lysates were separated on SDS-PAGE electrophoresis. Western blotting was performed according to the standard pro
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Rresponding gene knock-out mice. Processing of the new protein substrates by MMP-2 and MMP-9 identified here provides new mechanisms for gelatinase involvement in angiogenesis and carcinogenesis rather than extracellular matrix degradation (7, 51) and suggests new avenues for therapeutic intervention.Acknowledgments--We thank Dr. Wei Chen from the University of British Columbia Centre for Blood Re
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