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Nan (100 /ml) for 3 h at 37 in a 5 CO2 atmosphere. Cells were washed four times and autologous stimulated T cells were added onto HIV-exposed MDDC at a DC:T-cell ratio of 1:5. Each sample was performed in triplicate. Culture supernatants were harvested every 3 days and fresh medium was added. Supernatants were inactivated with 1 Triton X-100 and frozen at -20 . The viral production by T lympho

Represent the seeds in the study. The circles represent female sex workers working on the street and the squares those in indoor venues. Figure. Network of female sex workers who participated in the study according to workplace. Rio de Janeiro, RJ, 2009. (N = 601)of syphilis and HIV. The difference in syphilis serologic scars was almost 10 percentage points higher among those who worked on the str

D presentation--Antigen processing and presentation through the major histocompatibility complex (MHC) is a critical step in initiating effective cell-mediated adaptive immune response against pathogens. Downregulation of the cell surface MHC class I molecules is a key viral immune evasion strategy. KSHV encodes two zinc finger membrane proteins MIR1 and MIR2 (also called K3 and K5) which are E3 u

Tagonist `Compound A' [4-nitrobenzyl 1-(3(N-methylphenylsulfonamido)-3-phenylbutyl)piperidin-4-yl(vinyl)carbamate] [41,42] was kindly provided by Dr. Martin Springer at Merck Research Laboratories, Rahway, NJ. The anti-gp120 monoclonal antibody 48d, which recognizes an epitope that overlaps the coreceptor-binding site, was kindly provided by Dr. James Robinson (Tulane University Medical Center) [4

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S) and cells were lysed (1 Triton X-100 for 45 min at 37 ). Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of HIV-p24 antigen associated to cell lysates was determined using the HIV-1 p24 core profile ELISA. HIV-1 attachment on MDDC to assess the attachment of HIV-1 to MDDC, the cells were washed 2 times after 6 days of differentiation and seeded into 96-well cultu

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