D GIPZ lentiviral control vectors were also obtained from Thermo Scientific. To generate nonreplicating lentivirus stocks, lentiviral gene silencing constructs were cotransfected with pMD2.G and psPAX2 vectors (Addgene plasmid 12259 and Addgene plasmid 12260, respectively; deposited by Dr. Didier Trono) into packaging HEK 293-T cells. Cell supernatants containing nonreplicating lentiviral vectors

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Ity Nijmegen Medical Centre, Geert Grooteplein 28, Nijmegen, 6525 GA, The Netherlands Full list of author information is available at the end of the articleof breast cancers. Apart from breast epithelial tumor cells, ESR2 is also expressed in adjacent infiltrating lymphocytes, fibroblasts, and endothelial cells, all of which are known to influence tumor growth [3]. However, its precise role in bre

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