Erapy, and had received methadone or buprenorphine for 3 weeks at the OTP with no plans to discontinue. We also required verbal approval from participants' HIV providers and confirmation of active insurance coverage for ART. Exclusion criteria included ART dosed more frequently than twice daily, use of liquid medication, and use of a regimen that was predicted to have fewer than 1.5 active drugs

Erapy, and had received methadone or buprenorphine for 3 weeks at the OTP with no plans to discontinue. We also required verbal approval from participants' HIV providers and confirmation of active insurance coverage for ART. Exclusion criteria included ART dosed more frequently than twice daily, use of liquid medication, and use of a regimen that was predicted to have fewer than 1.5 active drugs

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Kplace was not reached, probably resulting in underestimation of HIV prevalence. This limitation does not apply to this study, as the sample was sufficient to achieve Markov equilibrium and the homophily effect did not affect the estimated HIV prevalence. The estimate was very close to what had been found previously,28 even using the procedure of post-stratification according to workplace. To conc

To current convention [46]). C34-Ig was produced and purified as previously described [47]. The CD4-Ig fusion protein consists of the first two N-terminal domains of the CD4 molecule and the Fc region of human IgG1. Purification was carried out as described for the C34-Ig molecule [47].mechanism of sCD4 neutralization [17,18]. Resistance to sCD4 may thus arise by a decreased affinity of the envelo

Us combinations of plasmids. Bar, 10 m. In the absence of a viral RNA, MS2-YFP had the appearance shown in Fig. 4A to C. (A) pFIVgag-CFP, pMS2-YFP, and pFIV -24. The nuclear envelope region (box, right panel) is shown enlarged below (scale bar, 5 m). (B) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. (C and D) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. Live-cell confocal imaging was performed 48 h a

Us combinations of plasmids. Bar, 10 m. In the absence of a viral RNA, MS2-YFP had the appearance shown in Fig. 4A to C. (A) pFIVgag-CFP, pMS2-YFP, and pFIV -24. The nuclear envelope region (box, right panel) is shown enlarged below (scale bar, 5 m). (B) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. (C and D) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. Live-cell confocal imaging was performed 48 h a

Ation MDDC are washed and 2 ?105 cells were adsorbed on a microscopy-adapted slide. Cells were incubated with or without HHA-TRITC or GNA-TRITC (200 /ml; Eylabs) at 4 for 30 min. Cells were washed with PBS 0.01 azide 0.5 BSA and then fixed with 1 paraformaldehyde. The coverslides were mounted in Mowiol (SigmaAldrich). Fluorescence analysis was performed with a Zeiss LSM510 confocal microscope