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Utant that we had earlier observed associating with ESCRT-I, the YLPM1 associated proteins, and CPSF6/7. When we purified Halo-TNIP2 196 ?46 associated proteins in the presence of Benzonase? an endonuclease that digests both DNA and RNA, we detected the bait proteins shown in Fig. 5A (light gray bars). There was no significant change in the relative amounts of the subunits of the ESCRT-I complex d

Tumors, such as COL1A1, COL4A1, FN1, LOXL1, TGFBI, TNC, and VIM. Furthermore, annexins (ANXA1, ANXA2, and ANXA5), which are also membrane proteins, were detected. Additionally, several metabolic enzymes were detected, including glycolytic enzymes (aldolase A, fructose-bisphosphate; aldolase C, fructose-bisphosphate; enolase 2; GAPDH; LDHA; LDHAL68; PKM2; and TPI). It is of note that the tumor-spec

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Organ-confined groups. C, Gene Ontology enrichment of the extracapsular (EC) and organ-confined (OC) direct-EPS.Generation of Candidate Shortlist--To select proteins for verification in a small, independent cohort of direct-EPS samples, we performed a systematic data-mining strategy using in-house and publicly available resources to minimize the panel of 133 proteins to a refined verification set

Es between two samples and allows a more precise quantitative analysis. We first compared tumor and wound in this analysis (Fig. 8). When tumor and wound were compared and two statistical tests were performed, 39 proteins were found to be overexpressed ( 2fold) and 44 proteins were less expressed ( 2-fold) after tumor implantation (supplemental Table S2). Among the proteins overexpressed were seve