Severe immunodeficiency at baseline: 10 children were classified as WHO stage 4; 8 as WHO stage 3; and one as WHO stage 2. Median weight-for-age Z-score was 22.67 (range 25.66 to 20.19) and 9 of 18 had a haemoglobin level of ,10 g/dL. Only five patients had a pre-ART CD4 cell count; the median absolute CD4 cell count was 311 cells/mm3 (range 245?26 cells/mm3) and the median CD4 (CD4 cell count di

S did not count as first-line failures. The decision to switch to a second-line bPI-containing regimen for first-line failure was based on clinical criteria in both groups (new/ recurrent WHO 4 event (per-protocol); or single or multiple WHO 3 events at clinician discretion), or confirmed CD4,100 cells/ mm3 on ART (,50 cells/mm3 before July 2006) for LCM. Switching was discouraged before 48 weeks

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Ge activity .500 U/mL (13/21 vs 1/ 14, p,0.005) compared with MBL2 O/O or O/A haplotypes (O refers to B,C or D alleles). (EPS) Figure S3 Endoglycosidases cleave N-linked glycans in HIV-EBOV GP. We preincubated HIV-EBOZ GP virion-like particles (12,000 pg/ml) with PNGase F or endo H (10,000 U/ml each) diluted in DMEM or with DMEM alone for 1 hour at 37uC. Viruses then underwent gel electrophoresis;

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F a CCR5-antagonist-based treatment4,5. Deep sequencing techniques can detect minor variants, especially ones that use CXCR46?. The 454 GS-Junior sequencing platform (Roche) can reliably predict HIV-1 tropism9?1. The Ion Torrent Personal Genome Machine has also been validated for tropism determination12. Another study evaluated the Illumina platform for determining HIV-1 drug resistance and HIV-1

Exposed to autoradiography film. High-mannose moieties were preserved only in untreated virus confirming that PNGase F and endo H effectively cleaved high-mannose residues on GPs of HIV-EBOV virion-like particles. (EPS) Figure S4 Thermolysin treatment of HIV-EBOV GP abrogates enhancement of infection by rhMBL in a thermolysin-concentration dependent manner. We preincubated HIV-EBOV GP virion-like