E analysis the differentially secreted proteins were in-gel digested into peptides and identified using LC-MS/MS. In GeLC-MS/MS the proteins were separated using 10 ?0 Tris-HCl gradient gel and visualized with silver-staining. To identify all the secreted proteins the lanes were cut into 15 pieces, and proteins were identified using a combination of in-gel digestion and LC-MS/MS analysis.Molecula